Available Datasets:

Chromosome Images for Segmentation

Chromosome Images for Classification

Automatic Karyotyping:

Segmentation

Classification


Automatic Karyotyping: Classification


E. Poletti, E. Grisan and A. Ruggeri. Automatic classification of chromosomes in Q-band images. Proc. 29th Annual International Conference of IEEE-EMBS, pp. 1911-4, IEEE, New York, 2008.


Chromosome karyotyping analysis is an important screening and diagnostic procedure routinely performed in clinical and cancer cytogenetic labs. Chromosome are first stained with a fluorescent dye, and then imaged through a microscope for subsequent analysis and classification. Each chromosome in the image has to be identified and assigned to one of 24 classes: the result is the so-called karyotype image in which all chromosomes in a cell are graphically arranged according to an international system for cytogenetic nomenclature (ISCN) classification.

      

The figure shows a typical PAL resolution (768 x 576, 8 bits/pixel) Qbanding prometaphase image and the corresponding karyotype. Individual chromosomes only appear as distinct bodies towards the end of the cell division cycle, at prophase, when they are long string-like objects, contracting and separating at metaphase, just before cell division. The intermediate stage of contraction between prophase and metaphase is called prometaphase.
Most of the studies aimed at the development of ytogenetics systems for analysis of banded chromosome preparations, have concentrated on metaphase chromosomes, avoiding the segmentation difficulties arising from touches and overlaps in the prophase and prometaphase cells.


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